LOCUS       40924_27638        4604 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pLD7-rrnP-MCS DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Hasunuma T, Miyazawa SI, Yoshimura S, Shinzaki Y, Tomizawa KI, 
            Shindo K, Choi SK, Misawa N, Miyake C.
  TITLE     Biosynthesis of astaxanthin in tobacco leaves by transplastomic 
            engineering
  JOURNAL   Plant J. (2008) In press
  PUBMED    18494855
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Hasunuma T, Miyake C.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2008) Contact:Tomohisa Hasunuma Research Institute
            of Innovative Technology for the Earth, Plant Research Group; 9-2 
            Kizugawadai, Kizugawa-shi, Kyoto 619-0292, Japan
REFERENCE   3  (bases 1 to 4604)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4604)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            (2008) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JAN-2008) Contact:Tomohisa Hasunuma Research Institute of 
            Innovative Technology for the Earth, Plant Research Group; 9-2 
            Kizugawadai, Kizugawa-shi, Kyoto 619-0292, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      2237..2353
                     /label=tobacco rrn promoter
                     /note="tobacco rrn promoter"
                     /regulatory_class="promoter"
     CDS             2378..3163
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      3592..3708
                     /label=tobacco rrn promoter
                     /note="tobacco rrn promoter"
                     /regulatory_class="promoter"
     promoter        complement(3961..3979)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3989..4005)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4147..4602
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctctta agctagcggc
     2221 cgctctagtt ggatttgctc ccccgccgtc gttcaatgag aatggataag aggctcgtgg
     2281 gattgacgtg agggggcagg gatggctata tttctgggag cgaactccgg gcgaatttga
     2341 agcgcttgga tacagttgta gggagggatc catggctcgt gaagcggtta tcgccgaagt
     2401 atcaactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     2461 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     2521 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     2581 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     2641 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     2701 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     2761 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     2821 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     2881 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     2941 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     3001 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     3061 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     3121 tgtccactac gtgaaaggcg agatcactaa ggtagttggc aaataactgc aggatcctgg
     3181 cctagtctat aggaggtttt gaaaagaaag gagcaataat cattttcttg ttctatcaag
     3241 agggtgctat tgctcctttc tttttttctt tttatttatt tactagtatt ttacttacat
     3301 agactttttt gtttacatta tagaaaaaga aggagaggtt attttcttgc atttattcat
     3361 gattgagtat tctattttga ttttgtattt gtttaaaatt gtagaaatag aacttgtttc
     3421 tcttcttgct aatgttacta tatctttttg attttttttt tccaaaaaaa aaatcaaatt
     3481 ttgacttctt cttatctctt atctttgaat atctcttatc tttgaaataa taatatcatt
     3541 gaaataagaa agaagagcta tattcgaagc ttaattaatc tagttggatt tgctcccccg
     3601 ccgtcgttca atgagaatgg ataagaggct cgtgggattg acgtgagggg gcagggatgg
     3661 ctatatttct gggagcgaac tccgggcgaa tttgaagcgc ttggatacag ttgtagggag
     3721 agatctcagt ctagagtggc tagcgacatc gatggcgaat tcgcccttcc gaagcttgaa
     3781 attcaattaa ggaaataaat taaggaaata caaaaagggg ggtagtcatt tgtatataac
     3841 tttgtatgac ttttctcttc tatttttttg tatttcctcc ctttcctttt ctatttgtat
     3901 ttttttatca ttgcttccat tgaattacta gtcgacaatt gcttaaggta cccaattcgc
     3961 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     4021 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     4081 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     4141 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4201 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4261 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4321 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4381 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4441 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4501 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4561 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttag
//
